Engineering Novel and Improved Biocatalysts by Cell Surface Display
نویسندگان
چکیده
منابع مشابه
Engineering Novel and Improved Biocatalysts by Cell Surface Display.
Biocatalysts, especially enzymes, have the ability to catalyze reactions with high product selectivity, utilize a broad range of substrates, and maintain activity at low temperature and pressure. Therefore, they represent a renewable, environmentally friendly alternative to conventional catalysts. Most current industrial-scale chemical production processes using biocatalysts employ soluble enzy...
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Cell-surface display is the expression of peptides and proteins on the surface of living cells by fusing them tofunctional components of cells which are exposed to the environment of cells. This strategy can be carriedout using different surface proteins of cells as anchoring motifs and different proteins from different sourcesas a passenger protein. It is a promising strategy...
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Yeast surface display (YSD) is a powerful tool for engineering the affinity, specificity, and stability of antibodies, as well as other proteins. Since first described in 1997 by Boder and Wittrup, YSD has been employed successfully in engineering a number of antibodies, as well as T-cell receptors. A recently reported large nonimmune single chain antibody library serves as a good starting poin...
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Enzymes produced from bacteria and eukaryotic organisms are presently being used for a large variety of different biotechnological applications. The rapidly increasing demand for enzymes which are active towards novel and often non-natural substrates has triggered the development of novel molecular biological methods of enzyme isolation and design. The metagenome approach is a cultivation-indep...
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ژورنال
عنوان ژورنال: Industrial & Engineering Chemistry Research
سال: 2015
ISSN: 0888-5885,1520-5045
DOI: 10.1021/ie504071f